| NucleoSpin® TriPrep |
RNA, DNA and protein isolation
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| Features |
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| Parallel isolation of RNA, DNA, and protein from undivided samples |
 • Convenient one-column preparation of RNA, DNA, and protein
• High quality RNA and DNA, ready to use for downstream applications
• High protein yield independent of protein size, localization, modification, etc.
• Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
• Complete kit, including: NucleoSpin® Filters (shredders) for optimal lysis,
rDNase for on-column DNA digestion, Protein Solving Buffer for solving all types of proteins |
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| Technology |
Silica-membrane technology |
| Format |
Mini spin columns |
| Sample material |
< 5 x 106 cells, <30 mg human/ animal Tissue, <100 mg plant
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RNA |
DNA |
Protein |
| Fragment size |
> 200 nt |
< 30 kbp |
15–300 kbp |
| Typical yield |
< 70 µg |
< 6 µg |
< 1200 µg |
| A260/A280 |
1.9–2.1
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1.7–1.9 |
– |
| Typical RIN (RNA integrity number) |
> 9 |
– |
– |
Elution volume (RNA and DNA)
Resolubilization volume (protein) |
40–120 µL |
100 µL |
10–100 µL |
| Preparation time |
30 min/6 preps |
35 min/6 preps (RNA+DNA) |
30 min/6 preps |
| Binding capacity |
200 µg |
10 µg* |
– |
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Applications**
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• Rapid purification of total RNA, DNA, and protein from small and precious samples – no sample splitting for
different isolations necessary
• Gene expression profiling, analysis of transgenic organisms, drug screening, genotyping
• Reliable interpretation of RNA, DNA, and protein amounts
• Good preservation of protein primary structure and posttranslational modifications (e.g., protein
phosphorylation) allows protein analysis without additional inhibitors (e.g., proteinase or phosphatase
inhibitors)
• Suitable for a broad spectrum of starting materials, including cultured cells, tissue, bacteria, yeast, and
plants
• RNA and DNA are ready to use for all typical downstream applications
• Proteins are ready to use for SDS-PAGE / Western blotting |
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* Binding capacity of DNA < 10 μg, strongly depending on RNA amount bound to the membrane.
** Kits to be used for research purposes only. DISTRIBUTION AND USE IN THE USA IS PROHIBITED FOR PATENT REASONS. |
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