KARS (C-term) Peptide
产品名称： KARS (C-term) Peptide
英文名称： KARS (C-term) Peptide
产品编号： P2856
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- 所在区域 : 台湾
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- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of KARS.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human KARS.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Crystal structure of tetrameric form of human lysyl-tRNA synthetase: Implications for multisynthetase complex formation.
Guo M, Ignatov M, Musier-Forsyth K, Schimmel P, Yang XL.Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2331-6. Epub 2008 Feb 13.
- 2.
- Critical role of helix 4 of HIV-1 capsid C-terminal domain in interactions with human lysyl-tRNA synthetase.
Kovaleski BJ, Kennedy R, Khorchid A, Kleiman L, Matsuo H, Musier-Forsyth K.J Biol Chem. 2007 Nov 2;282(44):32274-9. Epub 2007 Aug 27.
- 3.
- Role of HIV-1 Vpr-induced apoptosis on the release of mitochondrial lysyl-tRNA synthetase.
Kaminska M, Francin M, Shalak V, Mirande M.FEBS Lett. 2007 Jun 26;581(16):3105-10. Epub 2007 Jun 6.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of KARS polyclonal antibody (Cat # PAB3515) in Jurkat cell line lysates (35 ug/lane). KARS (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human colon carcinomareacted with KARS polyclonal antibody (Cat # PAB3515), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 3735
- Protein Accession#:
- NP_005539;Q15046
- Gene Name:
- KARS
- Gene Alias:
- KARS2,KIAA0070,KRS
- Gene Description:
- lysyl-tRNA synthetase
- Omim ID:
- 601421
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- lysine tRNA ligase
- Gene Pathway
- Related Disease