AURKC polyclonal antibody
产品名称： AURKC polyclonal antibody
英文名称： AURKC polyclonal antibody
产品编号： PAB4509
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
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- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of AURKC.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human AURKC.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunofluorescence (1:100-500)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3.
Kimura M, Matsuda Y, Yoshioka T, Okano Y.J Biol Chem. 1999 Mar 12;274(11):7334-40.
- 2.
- Cloning of STK13, a third human protein kinase related to Drosophila aurora and budding yeast Ipl1 that maps on chromosome 19q13.3-ter.
Bernard M, Sanseau P, Henry C, Couturier A, Prigent C.Genomics. 1998 Nov 1;53(3):406-9.
- 3.
- Protein kinase profile of sperm and eggs: cloning and characterization of two novel testis-specific protein kinases (AIE1, AIE2) related to yeast and fly chromosome segregation regulators.
Tseng TC, Chen SH, Hsu YP, Tang TK.DNA Cell Biol. 1998 Oct;17(10):823-33.
- Applications
- Western Blot (Cell lysate)
- The AURKC polyclonal antibody (Cat # PAB4509) is used in Western blot to detect AURKC in lysates of 293 cells expressing Flag tag (lane 1) , Flag-tagged AURKA (lane 2) , Flag-tagged AURKB (lane 3) , Flag-tagged AURKC (lane 4) , and in A-375 cell lysate (lane 5) .
- Immunofluorescence
- In the immunofluorescence experiment, staining of HeLa cells expressing GFP-AURKC is performed at different cellular mitotic stages with The AURKC polyclonal antibody (Cat # PAB4509) (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-AURKC merged to DAPI staining (column D). Data with Flagged Aurora is kindly provided by Drs . K . Sasai and S . Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).
- Entrez GeneID:
- 6795
- Protein Accession#:
- Q9UQB9
- Gene Name:
- AURKC
- Gene Alias:
- AIE2,AIK3,AurC,STK13,aurora-C
- Gene Description:
- aurora kinase C
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq
- Other Designations:
- aurora/IPL1-related kinase 3,aurora/IPL1/EG2 protein 2,serine/threonine kinase 13 (aurora/IPL1-like)
- Related Disease